Dracorhodin perchlorate inhibit high glucose-induced connective tissue growth factor formation in human mesangial cells / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of dracorhodin perchlorate on high glucose-induced connective tissue growth factor (CTGF) expression in human mesangial cells (HMC), and its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN).</p><p><b>METHOD</b>The HMC were divided into low glucose group (LG group, 5.5 mmol x L(-1) D-glucose), high glucose group (HG Group 25 mmol x L(-1) D-glucose), each group was located with dracorhodin perchlorate (7. 5 micromol x L(-1)) as comparison, and was examined at 24, 48 h. The expression of CTGF mRNA was assessed by semi quantatiue RT-PCR, and the expression of CTGF protein was assessed by Western blot.</p><p><b>RESULT</b>Compared to HG group, the expression of CTGF mRNA and protein were reduced in LG group after stimulating 24, 48 hours (P < 0.01). Compared to HG group, the expression of CTGF mRNA and protein were reduced in HG group by added with dracorhodin perchlorate after stimulating 24, 48 hours (P < 0.01).</p><p><b>CONCLUSION</b>Dracorhodin perchlorate can inhibit high glucose-induced connective tissue growth factor expression in human mesangial cells, and this may be its mechanism of prevention and treatment on renal fibrosis in diabetic nephropathy (DN).</p>