Lentivirus-mediated LOX-1-RNA interference attenuates oxidative stress-induced apoptosis in myocardial cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 165-168, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-265671
ABSTRACT
<p><b>OBJECTIVE</b>To construct a lectin-like oxidized low-density lipoprotein receptor (LOX-1) RNA interference (RNAi) lentivirus and explore the role of LOX-1 in H(2)O(2)-induced apoptosis of rat myocardial cells.</p><p><b>METHODS</b>LOX-1 shRNA sequence was synthesized and cloned into pLentiLox3.7 (pLL3.7) lentiviral vector to construct the lentiviral vector pLL3.7-LOX1. The lentiviral vectors (pLL3.7 and pLL3.7-LOX1) and the packaging vectors dR8.9 and VSVG were co-transfected into 293FT cells for packaging the lentivirus. H9C2 myocardial cells were infected by the lentiviruses to observe the inhibitory rate of LOX-1 on H(2)O(2)-induced apoptosis of H9C2 cells by RT-PCR, CCK-8, and Hochest33258 staining.</p><p><b>RESULTS</b>Double restriction enzyme digestion and DNA sequencing confirmed correct insertion of the sequence. Suppression of LOX-1 by the lentivirus attenuated H(2)O(2)-induced cell viability reduction and apoptosis in the myocardial cells (P<0.01).</p><p><b>CONCLUSION</b>LOX-1 activation may play an important role in H(2)O(2)-induced apoptosis of rat myocardial cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Cells, Cultured
/
Apoptosis
/
Lentivirus
/
Oxidative Stress
/
Cell Biology
/
Myocytes, Cardiac
/
RNA, Small Interfering
/
RNA Interference
/
Scavenger Receptors, Class E
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
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