Mouse melanoma cell line B16F10-derived conditioned medium inhibits sodium L-ascorbate-induced B16F10 cell apoptosis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 146-150, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-265675
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of mouse melanoma cell line B16F10-derived conditioned medium on the apoptosis of B16F10 cells.</p><p><b>METHODS</b>B16F10 cells were cultured in high-glucose DMEM in the presence of 10% fetal bovine serum, and upon cell confluence, the growth medium was replaced with serum-free high-glucose DMEM. After 8 h, the medium was collected and infiltrated to serve as the conditioned medium. B16F10 cells cultured in normal growth medium or the conditioned medium were exposed to 10 mmol/L sodium L-ascorbate, and the cell apoptosis was analyzed. The ingredients in the conditioned medium with relative molecular mass less or more than 5 000 were extracted to assess their effect on sodium L-ascorbate-induced cell apoptosis.</p><p><b>RESULTS</b>The conditioned medium for B16F10 cells significantly inhibited cell apoptosis induced by sodium L-ascorbate, and the effective ingredients in the medium showed a relative molecular mass below 5,000.</p><p><b>CONCLUSION</b>Mouse melanoma cell line B16F10-derived conditioned medium can suppress sodium L-ascorbate- induced apoptosis of B16F10 cells, and the ingredients with relative molecular mass less than 5 000 are responsible for this effect.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Ascorbic Acid
/
Melanoma, Experimental
/
Culture Media, Conditioned
/
Apoptosis
/
Cell Line, Tumor
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
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