Effects of Bushen Huoxue Fang on rat cardiac fibroblast proliferation and collagen production in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 122-124, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-265681
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Bushen Huoxue Fang on the proliferation of rat cardiac fibroblasts and collagen production in the cells.</p><p><b>METHODS</b>Rat cardiac fibroblasts were isolated and cultured in DMEM containing 10% (group A) or 20% (group B) or no (group C) serum from rats treated with Bushen Huoxue Fang, with cells cultured in DMEM containing 10% FBS as the control (group D). After 72 h of cell culture, the proliferation of the fibroblasts was detected using CCK-8 kit, and collagen mRNA and protein expressions were examined using RT-PCR and Western blotting, respectively.</p><p><b>RESULTS</b>Compared with that in groups C and D, the cell proliferation decreased significantly in groups A and B, and especially in the latter (P<0.05). RT-PCR demonstrated significant reductions of the mRNAs of type 1 and 3 collagens in groups A and B (P<0.05), and their protein levels were also significantly lowered (P<0.05).</p><p><b>CONCLUSION</b>Bushen Huoxue Fang can effectively inhibit the proliferation of rat cardiac fibroblasts and reduced collagen type 1 and 3 productions in the cells in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Fibrosis
/
Drugs, Chinese Herbal
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Cell Biology
/
Collagen Type I
/
Collagen Type III
/
Cell Proliferation
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
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