Endothelial dysfunction induced by high glucose is associated with decreased ATP-binding cassette transporter G1 expression / 南方医科大学学报
Journal of Southern Medical University
;
(12): 14-18, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-265706
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of ATP-binding cassette transporter G1 (ABCG1) in endothelial dysfunction induced by high glucose.</p><p><b>METHODS</b>Human aortic endothelial cells (HAECs) were incubated in the presence of 5.6 or 30 mmol/L glucose for 24-72 h with or without a 2-h pretreatment with the LXR agonist 22(R)-hydroxycholesterol. Real-time PCR and Western blotting were used to measure the mRNA and protein expressions of ABCG1; the intracellular cholesterol efflux and endothelial nitric oxide synthase (eNOS) activity were measured by scintillation counting.</p><p><b>RESULTS</b>High glucose time-dependently suppressed ABCG1 expression and cholesterol efflux to HDL in HAECs. High glucose also decreased eNOS activity. ABCG1 down-regulation induced by high glucose, along with decreased cholesterol efflux and eNOS activity, was abolished by treatment of the cells with the LXR agonist.</p><p><b>CONCLUSION</b>Endothelial dysfunction induced by high glucose is associated with decreased ABCG1 expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta
/
Pharmacology
/
Physiology
/
Down-Regulation
/
Cell Line
/
ATP-Binding Cassette Transporters
/
Cell Biology
/
Endothelial Cells
/
ATP Binding Cassette Transporter, Subfamily G, Member 1
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
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