Establishment of molecular beacon real-time PCR for detecting p53 gene single nucleotide polymorphism / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1875-1878, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-265763
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method based on molecular beacon real-time PCR for detecting single nucleotide polymorphisms (SNP) in codon 72 of scar-related p53 gene.</p><p><b>METHODS</b>Two fluorescence-labeled molecular beacon probes were synthesized targeting CCC/CGC SNP of p53 codon 72. The genomic DNA was extracted from the peripheral blood of 28 patients with keloid, and the CCC/CGC SNP of P53 gene codon 72 were assayed with molecular beacon real-time PCR. The results of SNP typing were compared with the results of reverse dot hybridization and confirmed by direct DNA sequencing.</p><p><b>RESULTS</b>The goodness of fit of this method was 100% in comparison with direct DNA sequencing, higher than that of reverse dot hybridization.</p><p><b>CONCLUSION</b>Molecular beacon real-time PCR is suitable for rapid clinical detection of SNPs in p53 gene.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Codon
/
Molecular Sequence Data
/
Base Sequence
/
Tumor Suppressor Protein p53
/
Polymorphism, Single Nucleotide
/
Real-Time Polymerase Chain Reaction
/
Genetics
/
Keloid
/
Methods
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
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