Role of silencing phosphatase of regenerationg liver-3 expression by microRNA interference in the growth of gastric cancer / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2534-2538, 2008.
Article
in English
| WPRIM
| ID: wpr-265901
ABSTRACT
<p><b>BACKGROUND</b>Accumulated evidences demonstrate that phosphatase of regeneration liver-3 (PRL-3) is associated with metastasis of multiple tumor types, and has been validated as a potential therapeutic target for metastasis. High expression of PRL-3 was implicated in lymph node metastasis of gastric cancer. In the present study, we investigated the role of silencing PRL-3 expression by microRNA (miRNA) interference in gastric cancer growth.</p><p><b>METHODS</b>RNA interference, mediated by recombinant lentivirus expressing artificial PRL-3 miRNA, was employed to knockdown PRL-3 expression in human SGC7901 gastric cancer cells. MTT assay and tumor implantation experiment were conducted to determine the role of PRL-3 in the proliferation of SGC7901 cells and tumor growth.</p><p><b>RESULTS</b>Transfection of recombinant lentivirus expressing artificial PRL-3 miRNA significantly suppressed the proliferation of SGC7901 cells in vitro. The implanted tumor size of the PRL-3 transfection group was (1.92 +/- 0.18) cm(3), significantly smaller compared with controls (P < 0.001).</p><p><b>CONCLUSIONS</b>Knockdown of PRL-3 significantly suppressed the proliferation of SGC7901 cells in vitro and tumor growth in vivo. PRL-3 plays a key role in the growth of gastric cancer. PRL-3 should be considered as a potential therapeutic target.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Stomach Neoplasms
/
Blotting, Western
/
Protein Tyrosine Phosphatases
/
Reverse Transcriptase Polymerase Chain Reaction
/
MicroRNAs
/
RNA Interference
/
Cell Line, Tumor
/
Cell Proliferation
/
Genetics
Limits:
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2008
Type:
Article
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