Changes of sphingolipids profiles after ischemia-reperfusion injury in the rat liver / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 3025-3031, 2009.
Article
in English
| WPRIM
| ID: wpr-265965
ABSTRACT
<p><b>BACKGROUND</b>Hepatic ischemia-reperfusion (I/R) injury occurs in many clinical procedures. The molecular mechanisms responsible for hepatic I/R injury however remain unknown. Sphingolipids, in particular ceramide, play a role in stress and death receptor-induced hepatocellular death, contributing to the progression of several liver diseases including liver I/R injury. In order to further define the role of sphingolipids in hepatic I/R, systemic analysis of sphingolipids after reperfusion is necessary.</p><p><b>METHODS</b>We investigated the lipidomic changes of sphingolipids in a rat model of warm hepatic I/R injury, by delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry (DE MALDI-TOF-MS).</p><p><b>RESULTS</b>The total amounts of ceramide and sphingomyelin and the intensity of most kinds of sphingolipids, mainly sphingomyelin, significantly increased at 1 hour after reperfusion (P < 0.05) and reached peaks at 6 hours after reperfusion (P < 0.01) compared to controls. Six new forms of ceramide and sphingomyelins appeared 6 hours after reperfusion, they were (m/z) 537.8, 555.7, 567.7, 583.8, 683.5 and 731.4 respectively. A ceramide-monohexoside (m/z) 804.4 (CMH(d181C221+Na)(+)) also increased after reperfusion and correlated with extent of liver injury after reperfursion.</p><p><b>CONCLUSIONS</b>Three main forms of sphingolipids, ceramide, sphingomyelin and ceramide-monohexoside, are related to hepatic I/R injury and provide a new perspective in understanding the mechanisms responsible for hepatic I/R injury.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Sphingolipids
/
Reperfusion Injury
/
Tumor Necrosis Factor-alpha
/
Rats, Sprague-Dawley
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Reverse Transcriptase Polymerase Chain Reaction
/
Genetics
/
Liver
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2009
Type:
Article
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