Generation and characterization of a cold-adapted attenuated live H3N2 subtype influenza virus vaccine candidate / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2880-2885, 2009.
Article
in English
| WPRIM
| ID: wpr-266022
ABSTRACT
<p><b>BACKGROUND</b>H3N2 subtype influenza A viruses have been identified in humans worldwide, raising concerns about their pandemic potential and prompting the development of candidate vaccines to protect humans against this subtype of influenza A virus. The aim of this study was to establish a system for rescuing of a cold-adapted high-yielding H3N2 subtype human influenza virus by reverse genetics.</p><p><b>METHODS</b>In order to generate better and safer vaccine candidate viruses, a cold-adapted high yielding reassortant H3N2 influenza A virus was genetically constructed by reverse genetics and was designated as rgAA-H3N2. The rgAA-H3N2 virus contained HA and NA genes from an epidemic strain A/Wisconsin/67/2005 (H3N2) in a background of internal genes derived from the master donor viruses (MDV), cold-adapted (ca), temperature sensitive (ts), live attenuated influenza virus strain A/Ann Arbor/6/60 (MDV-A).</p><p><b>RESULTS</b>In this presentation, the virus HA titer of rgAA-H3N2 in the allantoic fluid from infected embryonated eggs was as high as 11024. A fluorescent focus assay (FFU) was performed 24-36 hours post-infection using a specific antibody and bright staining was used for determining the virus titer. The allantoic fluid containing the recovered influenza virus was analyzed in a hemagglutination inhibition (HI) test and the specific inhibition was found.</p><p><b>CONCLUSION</b>The results mentioned above demonstrated that cold-adapted, attenuated reassortant H3N2 subtype influenza A virus was successfully generated, which laid a good foundation for the further related research.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Viral Proteins
/
Influenza Vaccines
/
Vaccines, Attenuated
/
Chlorocebus aethiops
/
Reassortant Viruses
/
COS Cells
/
Hemagglutinin Glycoproteins, Influenza Virus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Allergy and Immunology
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2009
Type:
Article
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