Molecular characteristics and antibiotic resistances of Vibrio cholerae O1 isolates in Hangzhou in 2009 / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 895-898, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-266077
ABSTRACT
<p><b>OBJECTIVE</b>To study the molecular characteristics and antibiotic resistances of Vibrio cholerae (V. cholerae) O1 isolates in Hangzhou in 2009.</p><p><b>METHODS</b>The virulence genes ctxA and tcpA of the thirty V. cholerae O1 isolates from 7 counties and districts of Hangzhou were detected by PCR. Pulsed-field gel electrophoresis (PFGE) was performed for molecular typing and similarity analysis. Antibiotic resistances of these isolates were measured by the Kirby-Bauer method.</p><p><b>RESULTS</b>Virulence gene analysis showed that 80.00% (24/30) of the genotype in V. cholerae isolates was ctxA- and tcpA+, 13.33% (4/30) was ctxA- and tcpA-, and 6.67% (2/30) was ctxA+ and tcpA+. Twenty-seven isolates tested were typed into 11 PFGE patterns (P1-P11). Twenty-three isolates with genotype ctxA- and tcpA+ were clustered into 7 PFGE patterns (P1-P7, termed P1-like cluster) with the similarity to be equal or greater than 91.4%, and 56.52%(13/23) of them belong to P1. 7 isolates with very high similarity (97.6%), belonging to P1 (6 isolates), and P2 (1 isolate), respectively, were collected from one foodborne disease outbreak. The resistant rates of the 24 isolates with genotype ctxA- and tcpA+ to ampicillin, tobramycin and amikacin were 20.83% (5/24), 4.17% (1/24) and 4.17% (1/24), respectively.</p><p><b>CONCLUSION</b>The genotype of the epidemic strains of V. cholerae O1 isolates in Hangzhou in 2009 with high similarity was ctxA- and tcpA+; The level of drug resistances of this kind of V. cholerae O1 isolates were not high.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
China
/
Cholera Toxin
/
Electrophoresis, Gel, Pulsed-Field
/
Drug Resistance, Bacterial
/
Vibrio cholerae O1
/
Molecular Typing
/
Genes, Bacterial
/
Genetics
/
Genotype
Limits:
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Preventive Medicine
Year:
2011
Type:
Article
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