Inhibitory effect of solanine on prostate cancer cell line PC-3 in vitro / 中华男科学杂志
National Journal of Andrology
;
(12): 284-287, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-266176
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanisms of the effects of solanine on human androgen-independent prostate cancer cell line PC-3 in vitro.</p><p><b>METHODS</b>PC-3 cells were treated with solanine at the concentration of 0, 30, 40 and 50 microg/ml, and the cell activity was measured by CCK-8 at 12, 24 and 48 hours after the treatment. At 24 hours, the cell cycle and apoptosis were detected by flow cytometry and fluorescence microscopy, and the protein expressions of I(kappa)B(alpha) and Bcl-2 determined by Western blot.</p><p><b>RESULTS</b>Solanine suppressed the growth of PC-3 cells in a dose- and time-dependent manner in vitro, with significant differences among different concentration and time groups (P < 0.05). The cycle of the PC-3 cells was arrested in the S phase (P < 0.05), with a significantly higher rate of apoptosis in the treated groups than in the controls (P < 0.05). The protein expression of I(kappa)B(alpha) was obviously up-regulated and that of Bcl-2 down-regulated in all the solanine concentration groups.</p><p><b>CONCLUSION</b>Solanine has an anti-prostate cancer effect by inhibiting PC-3 cell proliferation, arresting the S phase, inducing cell apoptosis, up-regulating the protein expression of I(kappa)B(alpha) and down-regulating that of Bcl-2.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Prostatic Neoplasms
/
Solanine
/
Cell Cycle
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
I-kappa B Proteins
/
Cell Line, Tumor
/
Cell Proliferation
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2011
Type:
Article
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