Estradiol stimulates proliferation of prostatic smooth muscle cells via estrogen receptor alpha and IGF1 / 中华男科学杂志
National Journal of Andrology
;
(12): 131-135, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-266200
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of estrogen receptor alpha (ERa) and insulin-like growth factor 1 (IGF1) on the proliferation of prostatic smooth muscle cells (PSMCs) in vitro.</p><p><b>METHODS</b>The ERalpha shRNA expression frame was subcloned to the pGSadeno adenovirus vector by homologous recombination technology to construct the pGSaaeno-ERalpha vector. After the mouse PSMCs were transfected in vitro by pGSaaeno-ERalpha, the mRNA and protein expression levels of ERalpha were detected by RT-PCR and Western blot respectively. The expression of IGF1 in the ERa-reduced cells was determined by Western blot 6 hours after treatment with 17beta-estradiol (E2) at 10(-8) mol/L. The post-transfection activity of estrogen or exogenous IGF1 in the proliferation of PSMCs was evaluated by MTT chlormetric analysis.</p><p><b>RESULTS</b>After treatment with E2, the proliferation of PSMCs and the expression of the IGF1 gene were significantly increased in the normal control group (P <0.05), but not obviously changed in the ERalpha-siRNA group (P> 0.05). And exogenous IGF1 failed to induce the proliferation of the ERalpha-reduced PSMCs.</p><p><b>CONCLUSION</b>E2 induces the expression of IGF1 via ERalpha, and IGFl, with the interaction of ERalpha, promotes the proliferation of PSMCs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Prostate
/
RNA, Messenger
/
Insulin-Like Growth Factor I
/
Cells, Cultured
/
Cell Biology
/
Myocytes, Smooth Muscle
/
Estrogen Receptor alpha
/
Cell Proliferation
/
Estradiol
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2011
Type:
Article
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