Antagonistic effect of myricetin on platelet activing factor / 药学学报
Acta Pharmaceutica Sinica
;
(12): 831-833, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-266574
ABSTRACT
<p><b>AIM</b>To study the antagonistic effect of myricetin on platelet activing factor (PAF).</p><p><b>METHODS</b>The specific binding of [3H] PAF to rabbit platelet receptor was investigated using radio ligand binding assay (RLBA). Platelet adhesion induced by PAF was measured with spectrophotometry. The elevation of inner free calcium concentration in rabbit polymorphonuclear leukocytes (PMNs) induced by PAF was assayed by Fura-2 fluorescent technique.</p><p><b>RESULTS</b>The specific binding inhibition potency of Myr was found to be concentration-dependent. The IC50 of Myr in [3H] PAF 1, 2 and 4 nmol.L-1 were 34.8, 85.7 and 118.6 mumol.L-1, respectively. The PAF induced reactions of rabbit platelet adhesion and PMNs inner free calcium concentration increase were inhibited by Myr in a dose-dependent manner. The IC50 of Myr to inhibit platelet adhesion was 13.1 mumol.L-1.</p><p><b>CONCLUSION</b>The specific receptor binding of PAF can be antagonized by myricetin.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Flavonoids
/
Platelet Aggregation Inhibitors
/
Platelet Activating Factor
/
Platelet Membrane Glycoproteins
/
Platelet Activation
/
Platelet Adhesiveness
/
Calcium
/
Receptors, G-Protein-Coupled
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS