Analysis of the response factors of different quinolones detected by evaporative light-scattering detector / 药学学报

ABSTRACT

<p><b>AIM</b>To analyze the response factors of different quinolone antibiotics detected by evaporative light-scattering detector (ELSD).</p><p><b>METHODS</b>The response factors of five different quinolones (enoxacin, levofloxacin, ciprofloxacin, lomefloxacin and gatifloxacin) detected by ELSD were determined by using a YMC-Pack ODS-AM cloumn (150 mm x 4.6 mm ID, 5 microns) as analytical column and 0.5% triethylamine (adjusting pH 2.5 with trifluoroacetic acid)-acetonitrile (4812) as mobile phase at a flow rate of 0.6 mL.min-1, the temperature of the drift tube was set at 117 degrees C, and the flow of carrier gas at 3.0 L.min-1. Detector responses (A) and the amount of injection of each substance (m) were fitted to the logarithmic regression log A = b log m + log a.</p><p><b>RESULTS</b>The linear regression equation obtained were enoxacin Y = 1.0799X + 2.7611, r2 = 0.9996; levofloxacin Y = 1.0913X + 2.7235, r2 = 0.9997; ciprofloxacin Y = 1.0828X + 2.7523, r2 = 0.9994; lomefloxacin Y = 1.0891X + 2.7391, r2 = 0.9993; gatifloxacin Y = 1.0878X + 2.7392, r2 = 0.9995. The differences between them were negligible.</p><p><b>CONCLUSION</b>Different quinolones can give the same responses with ELSD detection. So, the HPLC-ELSD methods can be applied to the determination of new substances by using another substance as reference.</p>