Effects of quercetin on platelet-endothelial cell adhesion and their expression of adhesion molecules / 药学学报
Acta Pharmaceutica Sinica
;
(12): 574-577, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-266634
ABSTRACT
<p><b>AIM</b>To observe the effect of quercetin (Que) on the adhesion of platelets to cultured endothelial cells and adhesion molecule expression by human umbilical vein endothelial cells (HUVEC) and platelets.</p><p><b>METHODS</b>[3H]-Adenine labeled platelets were incubated with HUVEC to investigate the effect of Que on adhesion of platelets to HUVEC. The number of platelets adhering to the HUVEC monolayer was determined by liquid scintillation spectroscopy. TNF-alpha induced HUVEC expression ICAM-1 and thrombin induced platelets expression of P-selectin were measured by flow cytometry.</p><p><b>RESULTS</b>Que (0.3-2.4 mumol.L-1) was shown to inhibit the increase of P-selectin expression of thrombin activated platelets. Pretreatment of HUVEC with tumor necrosis factor (TNF-alpha) significantly increased platelets adhesion to HUVEC and the expression ICAM-1. Que (0.6-2.4 mumol.L-1) inhibited this effect of TNF-alpha in a concentration-dependent manner.</p><p><b>CONCLUSION</b>Que can inhibit the adhesion of platelets to HUVEC and the expression of adhesion molecules (P-selectin and ICAM-1).</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Quercetin
/
Umbilical Veins
/
Blood Platelets
/
Endothelium, Vascular
/
Gene Expression
/
Cell Adhesion
/
Cells, Cultured
/
Intercellular Adhesion Molecule-1
/
P-Selectin
Limits:
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2003
Type:
Article
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