Your browser doesn't support javascript.
loading
Experimental chemotherapy against xenografts derived from multidrug resistant KBv200 cells and parental drug-sensitive KB cells in nude mice by annonaceous acetogenin 89-2 / 药学学报
Acta Pharmaceutica Sinica ; (12): 565-570, 2003.
Article in English | WPRIM | ID: wpr-266636
ABSTRACT
<p><b>AIM</b>Annonaceous acetogenin 89-2 was obtained from atemoya plant. To investigate the effect of 89-2 on experimental chemotherapy against xenografts derived from multidrug resistant KBv200 cells and parental drug-sensitive KB cells.</p><p><b>METHODS</b>Cytotoxicity was determined by tetrazolium (MTT) assay. The models of KB and KBv200 xenografts in nude mice were established to investigate the effect of 89-2 on experimental chemotherapy against cancer in vivo. Mechanistic experiments were conducted to examine the function of P-gp by Fura 2-AM assay.</p><p><b>RESULTS</b>The compound 89-2 showed potent cytotoxicity in KBv200 and KB cells, and the mean IC50 of 89-2 to KBv200 and KB cells was 48.7 and 64.6 nmol.L-1, respectively. The IC50 of 89-2 to multidrug resistant (MDR) cells was similar to that to the parental drug-sensitive cells (P < 0.05). In the models of KBv200 and KB cell xenografts in nude mice, 89-2 (0.90 mg.kg-1, q2d x 6) exhibited 52.3% and 56.5% in inhibiting the growth of xenografts, respectively. The toxicity was endurable. The intracellular accumulation of Fura-2 in KBv200 cells increased to 1.66, 2.03, and 2.74-fold, respectively, by addition of 12.8, 64 and 320 nmol.L-1 of 89-2.</p><p><b>CONCLUSION</b>Both MDR KBv200 cells and parental drug-sensitive KB cells were sensitive to the treatment of 89-2 in vitro and in vivo. The mechanism of overcoming MDR was associated with the decrease of P-gp function MDR cells.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Plants, Medicinal / 4-Butyrolactone / Drugs, Chinese Herbal / KB Cells / Cell Division / Chemistry / ATP Binding Cassette Transporter, Subfamily B, Member 1 / Drug Resistance, Multiple / Drug Resistance, Neoplasm Limits: Animals / Humans / Male Language: English Journal: Acta Pharmaceutica Sinica Year: 2003 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Plants, Medicinal / 4-Butyrolactone / Drugs, Chinese Herbal / KB Cells / Cell Division / Chemistry / ATP Binding Cassette Transporter, Subfamily B, Member 1 / Drug Resistance, Multiple / Drug Resistance, Neoplasm Limits: Animals / Humans / Male Language: English Journal: Acta Pharmaceutica Sinica Year: 2003 Type: Article