Resonance Rayleigh scattering spectra of berberine-tetraphenylboron association nanoparticle and its analytical application / 药学学报
Acta Pharmaceutica Sinica
; (12): 530-533, 2003.
Article
in Zh
| WPRIM
| ID: wpr-266644
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To study the relationship between resonance Rayleigh scattering(RRS) and the formation of association nanoparticle, and to develop a new and sensitive RRS method for the determination of trace berberine (BB).</p><p><b>METHODS</b>The association nanoparticle between BB and tetraphenylboron (TPB) was investigated by means of RRS, absorption spectral method and transmission electron microscope (TEM).</p><p><b>RESULTS</b>In pH 5.0 NaAc-HAc buffer solution, BB and TPB combine to BB-TPB association complex. The association complexes aggregate to (BB-TPB)n association nanoparticles, due to the strong hydrophobic force and Van der Waals force. It exhibits a resonance Rayleigh scattering peak at 470 nm, a maximum absorption peak at 368 nm. A new RRS method was proposed for the determination of 0.06 to 5.28 mg.L-1 BB in real samples with satisfactory results. The detection limit is 26 micrograms.L-1.</p><p><b>CONCLUSION</b>The TEM of (BBjAgp-TPBj + p)h composite association nanoparticles was observed by means of composite association nanoreaction both TPB- and BB+ or Ag+. The results indicated that the formation of (BB-TPB)n association nanoparticles and interface of solid-liquid results in the enhanced resonance light-scattering. This new RRS method showed high sensitivity, and had been successfully applied to the determination of BB in real samples.</p>
Full text:
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Index:
WPRIM
Main subject:
Scattering, Radiation
/
Spectrum Analysis
/
Tablets
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Tetraphenylborate
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Berberine
/
Drug Carriers
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Drugs, Chinese Herbal
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Chemistry
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Technology, Pharmaceutical
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Nanotechnology
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2003
Type:
Article