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Research on regulation function of γ-secretase inhibitor DAPT on the differentiation of neural precursor cell line / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 126-130, 2015.
Article in Chinese | WPRIM | ID: wpr-266714
ABSTRACT
This study aims to investigate the effect of γ-Secretase Inhibitor DAPT, (N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester), on the differentiation of neural precursor cells and the production of neurons in the neural precursor cell line GE6. GE6 was cultured in medium with 4 μmol/L DAPT added as the experimental group and the untreated medium separately as the control group. After 4 days of differentiation, we carried out the following experiments. We used immuno-fluorescent staining to observe the ratio of Tuj1, GFAP and O4 positive cells. We also used qRT-PCR to detect the effect of the DAPT on Tuj1 and GFAP mRNA transcription in the GE6. The results of immuno-fluorescent staining indicated that the Tuj1 ratio of experimental group was higher compared to that of the control group, but the GFAP and O4 ratio of experimental group was lower than that of the control group. The differences were statistically significant (P < 0.05). The result of qRT-PCR was in accordance with immunofluorescent staining results. It was well concluded that DAPT could promote the neurogenic differentiation of neural precursor cell line rather than leading to gliogenic differentiation. More neurons could be obtained for transplantation with the addition of DAPT.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Cell Differentiation / Cell Line / Dipeptides / Amyloid Precursor Protein Secretases / Neural Stem Cells / Neurons Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Cell Differentiation / Cell Line / Dipeptides / Amyloid Precursor Protein Secretases / Neural Stem Cells / Neurons Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2015 Type: Article