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Study on the anti-apopotosis induced by hypoxia/hypoglycemia and reoxygenation of panax notoginseng saponins in cultured rat hippocampal neurons / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-266819
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the inhibitory effects of Panax Notoginseng Saponins(PNS) on apoptosis induced by hypoxia/hypoglycemia and reoxygenation in cultured rat hippocampal neurons.</p><p><b>METHOD</b>Apoptosis were measured by flow cytometry, intracellular free calcium concentration([Ca2+]i) was measured with confocal laser scanning microscopy, morphological changes and neuronal necrosis were observed with fluorescence microscope, and meanwhile the leakage of lactic dehydrogenase(LDH) was measured.</p><p><b>RESULT</b>Hypoxia/hypoglycemia cultures for 5 hours and reoxygenation induced neuronal apoptosis and necrosis, and significantly increased neuronal [Ca2+]i and the leakage of LDH. The effects were increased with the extending time of reoxygenation. PNS has could significantly decrease the percentage of neuronal apoptosis and necrosis, and reduce neuronal [Ca2+]i and the leakage of LDH.</p><p><b>CONCLUSION</b>PNS has inhibitory effect on neuronal apoptosis. This effect might be related to its effect of decreasing intracellular free calcium concentration.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Plants, Medicinal / Rats / Cell Hypoxia / Cells, Cultured / Chemistry / Calcium / Rats, Wistar / Apoptosis Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Plants, Medicinal / Rats / Cell Hypoxia / Cells, Cultured / Chemistry / Calcium / Rats, Wistar / Apoptosis Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2003 Type: Article