Effects of Forsythiae Fructus on guinea pig ileum contractility in vitro / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study contractility of guinea pig ileum in vitro,and analyze the mechanism of anti-emetic effects of Lianqiao.</p><p><b>METHOD</b>Using emesis-relating agonists as drugs, the inhibitory effects of Lianqiao on guinea pig ileum contractility in vitro were observed in organ bath.</p><p><b>RESULT</b>Lianqiao could inhibit guinea pig ileum spontaneous contractions, reducing the tone of contractions dose-dependently. Acetylcholine (Ach), histamine (His), 5-hydroxytryptamine (5-HT) stimulated contractions of the guinea pig ileum, enhanced the tone and amplitude. All the three doses (10, 5, 2 g x L(-1)) of Lianqiao could suppress the contractility, significantly reduced the tone and amplitude of ileum contractions stimulated by drugs but not the frequency. Dopamine could inhibit the spontaneous contraction tone and amplitude of ileum; Both the large doses (10, 5 g x L(-1)) of Lianqiao could antagonise the inhibitory effect of DA, enhance the tone and amplitude. Small dose(2 g x L(-1)) had additive effects on tone of ileum contractions with DA,but enhanced the amplitude not the frequency.</p><p><b>CONCLUSION</b>Lianqiao have an inhibitory effect on guinea pig ileum contractions,the mechanism might be blocking M receptor, H1 receptor, 5-HT receptor and D2 receptor or directly suppressed ileum smooth muscle. The mechanisms of anti-emetic effect of Lianqiao needs further study.</p>