Inhibitory effect of E2F-1-silencing lentivirus vector on chemoresistance of subcutaneous human gastric cancer in nude mice / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 655-659, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-267481
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of E2F-1-silencing lentivirus vector on the growth and chemoresistance of subcutaneous human gastric cancer in nude mice.</p><p><b>METHODS</b>Thirty-six nude mice were inoculated subcutaneously with chemoresistant SGC-7901/DDP cells to establish subcutaneous tumor models of gastric carcinoma. The mice were randomly divided into E2F-1/RNAi-LV group, LV-scrRNAi group and PBS group (n = 12). E2F-1/RNAi-LV, LV-scrRNAi or PBS (0.1 ml per time) was injected into the mice, respectively, every two days. The nude mice received an intraperitoneal injection of cisplatin (25 mg/kg) every two days. The tumor volume was measured and histopathological changes of the tumors were observed by HE staining. The expressions of E2F-1, c-Myc, survivin, MDR1 and MRP were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Apoptosis in tumor xenografts was determined by in situ TUNEL labeling technique.</p><p><b>RESULTS</b>The mean tumor growth rate of the E2F-1/RNAi-LV group was significantly slower than that of the LV-scrRNAi and control groups (P < 0.05). The tumor volume of the E2F-1/RNAi-LV group was (745.13 ± 154.42)mm(3), significantly lower than that of the LV-scrRNAi and PBS groups (P < 0.05). Compared with that in the LV-scrRNAi and PBS groups, the expressions of mRNA and protein of E2F-1, c-Myc, survivin, MDR1 and MRP were significantly decreased in the E2F-1/RNAi-LV group (P < 0.05). The apoptotic rate in the E2F-1/RNAi-LV treatment group was (27.5 ± 9.7)%, significantly higher than (7.0 ± 1.1)% in the LV-scrRNAi group and (7.3 ± 1.2)% in the PBS group (P < 0.05).</p><p><b>CONCLUSION</b>Intra-tumoral injection of E2F-1/RNAi-LV shows significantly inhibitory effect on the tumor growth and chemoresistance of subcutaneous human gastric cancer in nude mice.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Repressor Proteins
/
Stomach Neoplasms
/
RNA, Messenger
/
Transfection
/
Random Allocation
/
Proto-Oncogene Proteins c-myc
/
Cisplatin
/
Apoptosis
Type of study:
Controlled clinical trial
/
Prognostic study
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2013
Type:
Article
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