Effect of omeprazole on gene expression profile of human umbilical vein endothelial cell line and bioinformatics analysis / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To characterize the effect of omeprazole on the spectrum of gene expression in the cultured human umbilical vein endothelial cell (HUVEC) line (EA.hy926), and explore the underlying molecular mechanism.</p><p><b>METHODS</b>Affymetrix U133 plus2.0 oligonucleotide microarray was used to detect the alteration in the gene expression profiles induced by 1×10(-5) mol/L omeprazole in HUVECs. Real-time PCR was employed to verify the results of selected differentially expressed genes, and Western blotting was performed to test the expression levels of the related proteins.</p><p><b>RESULTS</b>A total of 282 genes were found to show at least 1.5-fold changes in EA.hy926 cells after treatment with omeprazole for 48 h, including 236 up-regulated and 46 down-regulated ones. These genes were involved in the regulation of transcription, inflammatory response, immune response, cell adherence, anti-apoptosis, and signal transduction.</p><p><b>CONCLUSION</b>Omeprazole modulates the function of endothelial cells by regulating the gene expression profiles of multiple pathways.</p>