Mechanisms of Chansu Injection in reversing multidrug resistance of HL60/ADM cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 337-340, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-267605
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanisms underlying the effect of Chansu Injection (CHS) in reversing multi-drug resistance (MDR) of HL60/ADM cells.</p><p><b>METHODS</b>MTT assay was used to investigate the effect of CHS on adramycin (ADM) sensitivity of HL-60/ADM cells. Flow cytometry was used to observe the effect of CHS on the cell cycle of HL60/ADM cell. The expressions of NF-κB, MRP, GST-π, and iNOS were detected by immunocytochemistry.</p><p><b>RESULTS</b>Treatment with CHS lowered the IC(50) of ADM in HL60/ADM cells from 34.1971 µmol/L to 17.4393 µmol/L, and caused an increase in G(0)/G1 and G(2)/M phase cells with decreased S phase cells. CHS decreased the expressions of MRP mRNA and GST-π and MRP proteins but increased the expressions of iNOS and NF-κB proteins in the cells.</p><p><b>CONCLUSION</b>CHS can partly reverse MDR in HL60/ADM cells possibly by down-regulating MRP and GST-π, up-regulating NF-κB and iNOS, and promoting cell apoptosis, thereby increase ADM sensitivity of HL-60/ADM cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Bufanolides
/
Doxorubicin
/
Drug Resistance, Multiple
/
HL-60 Cells
/
Drug Resistance, Neoplasm
/
Amphibian Venoms
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
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