Clinical application of DNA sequencing for detecting point mutations in hepatitis B virus associated with drug resistance / 南方医科大学学报
Journal of Southern Medical University
;
(12): 277-279, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-267617
ABSTRACT
<p><b>OBJECTIVE</b>[corrected] To assess the specificity and applicability of direct PCR sequencing in the detection of point mutations in hepatitis B virus (HBV) associated with drug resistance.</p><p><b>METHODS</b>Serum samples were obtained from 120 patients with hepatitis B treated with nucleoside analogus for at least 2 years to detect the point mutations in HBV genome in association with drug resistance using nested PCR and direct DNA sequencing.</p><p><b>RESULTS</b>Forty out of the 120 patients were found to have one or two point mutations associated with drug resistance, including 17 with L180M and M204V/I mutations (42.5%), 10 with M204V/I mutation (25%), 8 with N236T mutation (20%), 3 with L180M mutation (7.5%), and 1 with both A181V/T and N236T mutations (2.5%), and 1 with A181V/T mutation(2.5%).</p><p><b>CONCLUSION</b>DNA sequencing is a good method to detect all known point mutations associated with HBV drug resistance.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Hepatitis B virus
/
Polymerase Chain Reaction
/
Sequence Analysis, DNA
/
Point Mutation
/
Hepatitis B, Chronic
/
Drug Resistance, Viral
/
Therapeutic Uses
/
Drug Therapy
/
Genetics
Limits:
Adolescent
/
Adult
/
Aged
/
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS