Determination of rat serum emodin level by microemulsion liquid chromatography with direct sample loading / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2759-2761, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-267688
ABSTRACT
<p><b>OBJECTIVE</b>To establish a microemulsion liquid chromatography system with direct sample loading for determining the serum level of emodin in rats.</p><p><b>METHODS</b>The separation was performed on C₁₈ column (Hypersil BDS, 5 µm,150 mm×4.6 mm) with the microemulsion mobile phase consisting of 3.3% (w/V) SDS, 6.6% (V/V) n-butyl alcohol, and 1.0% (V/V) octane and water. The flow rate was 1.0 ml/min and the detection wavelength was 254 nm.</p><p><b>RESULTS</b>The linear range of emodin detection was 0.333-5.32 µg/ml. The average recovery was 99.65% with a RSD of 3.60%. The limit of quantification was 0.1386 µg/mL.</p><p><b>CONCLUSION</b>Microemulsion liquid chromatography system with direct sample loading allows simple, accurate and rapid determination of emodin in rat serum.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Chemistry
/
Chromatography, Liquid
/
Emodin
/
Rats, Sprague-Dawley
/
Serum
/
Methods
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
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