Construction of a lentivirus interfering vector targeting Cyc1 and its interfering efficiency in nasopharyngeal carcinoma cells / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the expression of Cyc1 in nasopharyngeal carcinoma (NPC) and evaluate the interfering efficiency of a lentivirus interfering vector targeting Cyc1 in NPC cells.</p><p><b>METHODS</b>Microarray technique was used to examine the expression of Cyc1 in NPC tissues. Real-time PCR was utilized to confirm the high expression of Cyc1 in NPC tissues and NPC cell lines. The recombinant Cyc1 shRNA-expressing plasmid (pLentiU6/Cyc1-shRNA) was stably transfected into NPC cells, and the interfering efficiency against Cyc1 was evaluated by quantitative RT-PCR.</p><p><b>RESULTS</b>The result of microarray showed that Cyc1 was highly expressed in NPC tissues compared to noncancerous nasopharyngeal tissues, as confirmed by Real-time PCR. All of the 8 NPC cells showed a high expression of Cyc1, among which 5-8F cells showed the highest expression. Sequence analysis indicated that the recombinant plasmid pLentiU6/Cyc1-shRNA was successfully constructed and could significantly and stably suppress the expression of Cyc1 in NPC cells.</p><p><b>CONCLUSION</b>Cyc1 is highly expressed in NPC cells. The lentivirus vector constructed can markedly inhibit the expression of Cyc1 in NPC cells, which provides assistance in the investigation of the function and molecular mechanism of Cyc1 in NPC.</p>