Asymmetric dimethylarginine upregulates the expression of ACAT-1 in THP-1 macrophage-derived foam cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2613-2618, 2010.
Article
in English
| WPRIM
| ID: wpr-267726
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of asymmetric dimethylarginine (ADMA) on ACAT-1 expression and cholesterol content in THP-1-derived macrophages and foam cells.</p><p><b>METHODS</b>THP-1 cells were induced to differentiate into macrophages and further into foam cells. The macrophages and foam cells were exposed to different concentrations (0, 3.75, 7.5, 15, and 30 µmol/L) of ADMA for varying time lengths (6, 12, and 24 h), and the changes in ACAT-1 mRNA and protein levels in the cells were measured with RT-PCR and Western blotting. The cellular cholesterol content was measured with enzyme-linked colorimetry assay.</p><p><b>RESULTS</b>In THP-1-derived macrophages and foam cells, the expression levels of ACAT-1 mRNA and protein and cellular cholesterol content increased significantly in response to ADMA treatment in a time- and concentration-dependent manner.</p><p><b>CONCLUSION</b>ADMA may play an important role in inducing foam cell formation from macrophages. ACAT-1 inhibition targeting the macrophages and foam cells may serve as a potential therapeutic target in the treatment of atherosclerosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Acetyl-CoA C-Acetyltransferase
/
Arginine
/
RNA, Messenger
/
Monocytes
/
Up-Regulation
/
Cell Line
/
Cholesterol
/
Cell Biology
/
Foam Cells
Limits:
Humans
Language:
English
Journal:
Journal of Southern Medical University
Year:
2010
Type:
Article
Similar
MEDLINE
...
LILACS
LIS