Your browser doesn't support javascript.
loading
Effects of some traditional Chinese drugs on Mdr1 gene and its expression product in K562/A02 cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 785-789, 2008.
Article in Chinese | WPRIM | ID: wpr-267888
ABSTRACT
This study was purposed to investigate the effects of angelicasinensis (Oliv) Diel compound injection, vauqueline, ephedrine and strychnine on human erythroleukemia multidrug resistance (MDR) K562/A02 cell line Mdr1 gene and p-glycoprotein. The MTT and trypan blue methods were used to analyze the cytotoxic effect of above-mentioned traditional Chinese drug; the expressions of K562/A02 cells Mdr1 gene and p-glycoprotein were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and Western blot respectively. The results showed that after K562/A02 cell were treated with angelicasinensis (Oliv) diels compound injection, vauqueline and ephedrine, mRNA transcription of Mdr1 gene was reduced significantly (p < 0.01); the expression of P-gp also decreased (p < 0.01). The expression level P-gp in group treated with vauqueline was the lowest, but the Mdr1 mRNA level and expression of P-gp of K562/A02 cells treated with strychnine did not obviously changed. It is concluded that angelicasinensis (Oliv) diels compound injection, vauqueline, ephedrine can partly reverse the multidrug resistance of K562/A02 cells, the down-regulation of Mdr1 gene causing decrease of p-glycoprotein expression may be one of the MDR reversal mechanisms in K562/A02 cells.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / RNA, Messenger / Drugs, Chinese Herbal / Doxorubicin / Down-Regulation / ATP Binding Cassette Transporter, Subfamily B, Member 1 / Drug Resistance, Multiple / Drug Resistance, Neoplasm / K562 Cells / Genetics Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2008 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / RNA, Messenger / Drugs, Chinese Herbal / Doxorubicin / Down-Regulation / ATP Binding Cassette Transporter, Subfamily B, Member 1 / Drug Resistance, Multiple / Drug Resistance, Neoplasm / K562 Cells / Genetics Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2008 Type: Article