Effect of hyperbaric oxygen on acute graft-versus-host disease after allogeneic bone marrow transplantation / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 623-626, 2008.
Article
in English
| WPRIM
| ID: wpr-267923
ABSTRACT
The objective of this study was to investigate the function and mechanism of hyperbaric oxygen (HBO) in antagonizing acute graft-versus-host disease (aGVHD) and improving the rate of survival. The lethally irradiated C57BL/6 recipients were injected with bone marrow and lymphocyte of spleen from BALB/c donors and were treated with HBO, cyclosporine A (CsA) and methotrexate (MTX). T lymphocytes and subsets, adhesion molecules and cytokines were detected by flow cytometry, ELISA and RT-PCR respectively. The results showed that the survival rate in HBO group was much higher than that in allogenetic bone marrow transplantation (allo-BMT) group and CsA + MTX group; the numbers of CD3(+), CD4(+), CD8(+), CD4(+)CD11a(+), CD4(+)CD18(+), CD8(+)CD11a(+), CD8(+)CD18(+) lymphocytes in spleen were decreased markedly by HBO and CsA + MTX (p < 0.05); the levels of IL-2 and TNFalpha mRNA and their serum concentrations in HBO group were much lower than those in allo-BMT group but were higher than those in CsA + MTX group; the levels of IL-4 and IL-10 mRNA in HBO group were much higher than those in allo-BMT group and CsA + MTX group. It is concluded that HBO has more remarkable advantage in improving the rate of survival than CsA + MTX, its mechanism of anti-aGVHD is tightly correlated with the transform of T cell and its subsets and the expression of adhesion molecules and cytokines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Therapeutics
/
T-Lymphocytes
/
Acute Disease
/
Cytokines
/
Whole-Body Irradiation
/
Bone Marrow Transplantation
/
Lymphocyte Transfusion
/
Allergy and Immunology
/
Graft vs Host Disease
/
Hyperbaric Oxygenation
Limits:
Animals
Language:
English
Journal:
Journal of Experimental Hematology
Year:
2008
Type:
Article
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