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Comparative analysis of naive and memory CD4(+) and CD8(+) T-cell subsets in rhG-CSF mobilized peripheral blood and steady-state bone marrow / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 605-609, 2008.
Article in Chinese | WPRIM | ID: wpr-267927
ABSTRACT
The study was aimed to analyze the difference of naive and memory CD4(+) and CD8(+) T-cell subsets between steady-state bone marrow (SS-BM) and recombinant human granulocyte colony-stimulating factor (rhG-CSF) mobilized peripheral blood grafts (G-PB). Four CD4(+) and CD8(+) T-cell subsets classified according to the expression of CD45RA and CD62L were determined by three-color flow cytometry. The results showed that the percentage of CD4(+), CD8(+) T-cell subsets and the ratios of CD4/CD8 in G-PB were significantly higher than those in SS-BM (p < 0.05). The percentage of CD4(+) naive T-cells in G-PB was significantly lower than that in SS-BM (p < 0.001). As compared with SS-BM, the percentage of CD4(+) effector memory T-cells was significantly high in G-PB (p < 0.001). There were no significant differences in the percentages of the four CD8(+) T-cell subsets between SS-BM and G-PB (p > 0.05). The percentage of CD4(+)CD62L(+) T-cells in G-PB was significantly lower than that in SS-BM (p = 0.001). The absolute numbers of CD4(+) and CD8(+) T-cell subsets, the eight naive and memory CD4(+) and CD8(+) T-cell subsets were significantly higher in G-PB than those in SS-BM (p < 0.001). It is concluded that the difference of naive and memory CD4(+) and CD8(+) subsets between G-PB and SS-BM may partially explain why the incidence and severity of acute graft-versus-host disease (GVHD) was similar and the incidence of chronic GVHD was different after transplantation with SS-BM or G-PB.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Recombinant Proteins / CD4-Positive T-Lymphocytes / Granulocyte Colony-Stimulating Factor / Bone Marrow Transplantation / CD4-CD8 Ratio / CD8-Positive T-Lymphocytes / Hematopoietic Stem Cell Mobilization / Peripheral Blood Stem Cell Transplantation / Therapeutic Uses / Allergy and Immunology Limits: Adolescent / Adult / Aged / Female / Humans / Male Language: Chinese Journal: Journal of Experimental Hematology Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Recombinant Proteins / CD4-Positive T-Lymphocytes / Granulocyte Colony-Stimulating Factor / Bone Marrow Transplantation / CD4-CD8 Ratio / CD8-Positive T-Lymphocytes / Hematopoietic Stem Cell Mobilization / Peripheral Blood Stem Cell Transplantation / Therapeutic Uses / Allergy and Immunology Limits: Adolescent / Adult / Aged / Female / Humans / Male Language: Chinese Journal: Journal of Experimental Hematology Year: 2008 Type: Article