Effects of hepatitis B virus on human semen parameters and sperm DNA integrity / 中华男科学杂志
National Journal of Andrology
;
(12): 896-898, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-268036
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of hepatitis B virus (HBV) in semen on human semen parameters and sperm DNA integrity.</p><p><b>METHODS</b>We detected HBV DNA in the semen samples of 153 HBsAg-seropositive patients by real-time fluorescence quantitative PCR and calculated the sperm nuclear DNA fragmentation index (DFI) by sperm chromatin dispersion (SCD) assay. We compared the semen parameters between the HBV DNA-positive group (A, n = 43) and HBV DNA-negative group (B, n = 110) and analyzed the correlation of sperm DFI with the number of HBV DNA copies in the semen.</p><p><b>RESULTS</b>HBV DNA was detected in 43 (28.1%) of the 153 semen samples. No statistically significant differences were observed in age, semen volume and sperm concentration between groups A and B (P >0.05). Compared with group B, group A showed significantly decreased sperm viability ([58.0 +/- 18.8]% vs [51.4 +/-17.1]%, P<0.05), progressively motile sperm ([29.6 +/- 13.3]% vs [24.5 +/- 10.1]%, P<0.05), average straight-line velocity ([23.7 +/- 4.0] microm/s vs [19.9 +/- 4.5 ] microm/s, P<0.01) and average path velocity ([26.5 +/- 7.0] microm/s vs [23.4 +/- 5.3] microm/s, P<0.01), but remarkably decreased sperm DFI ([19.3 +/- 8.0]% vs [24.2 +/- 9.4]%, P<0.01). The number of HBV DNA copies in semen exhibited a significant positive correlation with sperm DFI (r = 0.819, P < 0.01).</p><p><b>CONCLUSION</b>HBV DNA in semen is not significantly associated with the number of sperm, but may affect sperm viability, velocity and DFI. There is a load-effect relationship between the number of HBV DNA copies in semen and sperm nuclear DNA integrity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sperm Count
/
Spermatozoa
/
Virology
/
Blood
/
DNA, Viral
/
Hepatitis B virus
/
DNA Fragmentation
/
Semen Analysis
/
Genetics
/
Hepatitis B Surface Antigens
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2013
Type:
Article
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