Construction of eukaryotic expression vector for VEGF165 gene and its expression in rat bladder smooth muscle cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 654-656, 2007.
Article
in Zh
| WPRIM
| ID: wpr-268055
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct an eukaryotic expression vector for vascular endothelial growth factor (VEGF) 165 gene and obtain VEGF expression in rat bladder smooth muscle cells.</p><p><b>METHODS</b>VEGF165 cDNA was cloned into the eukaryotic expression vector pcDNA3.1(-), and the resultant recombinant vector pcDNA3.1(-)/VEGF165 was transfected into the rat bladder smooth muscle cells by electroporation. VEGF expression in the cells was determined by RT-PCR and immunofluoresence assay, and the biological activity of VEGF in the supernant of the transinfected cell culture was tested by MTT assay.</p><p><b>RESULTS AND CONCLUSION</b>VEGF expression was obtained in the transinfected cells, and the supernant of the transinfected cell cultures stimulated the proliferation of the endothelial cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Urinary Bladder
/
Transfection
/
Gene Expression
/
Cell Line
/
Cells, Cultured
/
Fluorescent Antibody Technique
/
Cloning, Molecular
/
Culture Media, Conditioned
/
DNA, Complementary
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article