Differential expression of isocitrate lyase in P. marneffei phagocytized by nonstimulated and stimulated murine macrophages / 南方医科大学学报
Journal of Southern Medical University
;
(12): 631-633, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-268062
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differential expression of isocitrate lyase in Penicillium marneffei phagocytized by nonstimulated and stimulated murine macrophages, and explore the role of glyoxylate pathway in pathogenesis of Penicilliosis marneffei.</p><p><b>METHODS</b>Penicillium marneffei conidia and Raw264.7 cells were incubated in 16 cultures, which were divided to 4 groups for treatment with N-monomethyl-L-arginine (LNMMA, CI group), murine interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS) (T group), IFN-gamma plus LPS and LNMMA (TI group), or the same volume of culture medium (C group). The transcriptional levels of isocitrate lyase were detected using real-time RT-PCR, and its expression levels detected biochemically.</p><p><b>RESULTS</b>The transcriptional levels of isocitrate lyase in C, CI, T, TI groups were 1.00, 1.42, 33.09, and 74.88 (P<0.05), while the expression levels were 0.06, 0.07, 0.18, and 0.93, respectively (P<0.05). The content of nitric oxide in T group was significantly higher than that in the other groups (P<0.01), but the CFU of T group was the lowest (P<0.01).</p><p><b>CONCLUSION</b>Reactive nitrogen intermediates induced by stimulated murine macrophages restrain the expression of isocitrate lyase of Penicillium marneffei and development of Penicillium marneffei, in which process the glyoxylate pathway may play an important role.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Penicillium
/
Phagocytosis
/
Pharmacology
/
Physiology
/
Fungal Proteins
/
Gene Expression Regulation, Enzymologic
/
Gene Expression Regulation, Fungal
/
Cell Line
/
Lipopolysaccharides
/
Interferon-gamma
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS