Sinerem labeling and MRI tracking of neural stem cells in vivo and in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 611-615, 2007.
Article
in Zh
| WPRIM
| ID: wpr-268067
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To label rat neural stem cells (NSCs) with the complex of Sinerem, the ultrasmall superparamagnetic iron oxide (USPIO), and poly-L-lysine (PLL), and evaluate the feasibility of tracking the labeled cells with magnetic resonance imaging (MRI) in vitro and in vivo.</p><p><b>METHODS</b>Sinerem was incubated with PLL to obtain the complex of Sinerem-PLL. The mesenchymal stem cells (MSCs) isolated from the bone marrow of SD rats were cultured and induced to differentiate into the neural stem cells. The second-passage cells were cultured overnight with the Sinerem-PLL complex, after which Prussian blue staining and transmission electron microscopy were performed to observe the nanoparticles in the cytoplasm. Cell apoptosis assay was performed to assess the cell viability 1 day, 1 week, and 2 weeks after the labeling. Cell tracking with 4.7 MR system was carried out in vivo and in vitro using T(2)WI and T(2)*WI sequences.</p><p><b>RESULTS</b>The NSCs could be effectively labeled with Sinerem-PLL complex with the labeling efficiency exceeding 95%. Prussian blue staining showed numerous blue iron particles in the cytoplasm, and under transmission electron microscope, these particles accumulated in the endosomes/lysosomes. The labeling did not significantly affect the cell viability and proliferation. Remarkable low signal density changes of the labeled cells was seen on T(2)WI and T(2)*WI in vivo and in vitro.</p><p><b>CONCLUSION</b>NSCs can be effectively labeled with Sinerem-PLL complex, and MRI can be used to track the labeled cells in vivo and in vitro.</p>
Full text:
1
Index:
WPRIM
Main subject:
Polylysine
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Endosomes
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Stem Cells
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Time Factors
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Magnetic Resonance Imaging
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Cell Differentiation
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Cells, Cultured
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Dextrans
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Rats, Sprague-Dawley
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Cell Biology
Limits:
Animals
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2007
Type:
Article