Analysis of aristolochic acid A from the aerial and underground parts of Asarum by UPLC-UV / 药学学报
Acta Pharmaceutica Sinica
;
(12): 221-223, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-268142
ABSTRACT
To develop an UPLC-UV method for the determination of aristolochic acid A in the aerial and underground parts of Asarum, an AcquityUPLC HSST3 column (50 mm x 2.1 mm ID, 1.8 microm) was used with the mobile phase of acetonitrile-0.1% FA and eluted in gradient mode. The flow rate was 0.5 mL min(-1), the detection wavelength was 251 nm, and the column temperature was 40 degrees C. The calibration curve was linear in the range of 18 - 1450 ng of aristolochic acid A with correlation coefficient of 0. 9999 and the minimum detective concentration was 18.25 ng mL (-1). The method developed is accurate and reproducible, and can be applied in the determination of aristolochic acid A in Asarum.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Spectrophotometry, Ultraviolet
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Plant Roots
/
Asarum
/
Plant Components, Aerial
/
Aristolochic Acids
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2008
Type:
Article
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