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Analysis of aristolochic acid A from the aerial and underground parts of Asarum by UPLC-UV / 药学学报
Acta Pharmaceutica Sinica ; (12): 221-223, 2008.
Article in Chinese | WPRIM | ID: wpr-268142
ABSTRACT
To develop an UPLC-UV method for the determination of aristolochic acid A in the aerial and underground parts of Asarum, an AcquityUPLC HSST3 column (50 mm x 2.1 mm ID, 1.8 microm) was used with the mobile phase of acetonitrile-0.1% FA and eluted in gradient mode. The flow rate was 0.5 mL min(-1), the detection wavelength was 251 nm, and the column temperature was 40 degrees C. The calibration curve was linear in the range of 18 - 1450 ng of aristolochic acid A with correlation coefficient of 0. 9999 and the minimum detective concentration was 18.25 ng mL (-1). The method developed is accurate and reproducible, and can be applied in the determination of aristolochic acid A in Asarum.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Spectrophotometry, Ultraviolet / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Sensitivity and Specificity / Plant Roots / Asarum / Plant Components, Aerial / Aristolochic Acids Type of study: Diagnostic study Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plants, Medicinal / Spectrophotometry, Ultraviolet / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Sensitivity and Specificity / Plant Roots / Asarum / Plant Components, Aerial / Aristolochic Acids Type of study: Diagnostic study Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2008 Type: Article