Tissue distribution and excretion of 5-fluorouracil from indomethacin 5-fluorouracil-1-ylmethylester in rats / 药学学报
Acta Pharmaceutica Sinica
;
(12): 81-85, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-268167
ABSTRACT
To study the tissue distribution and excretion of indomethacin 5-fluorouracil-1-ylmethyl ester (IFM) metabolite 5-fluorouracil in rats, an accurate and specific high performance liquid chromatography method for quantifying IFM in rat plasma and tissues was developed. Biological samples were prepared by liquid-liquid extraction and separated on a Diamonsil C18 column (250 mm x 4.6 mm ID, 5 microm). The mobile phase for tissue samples, plasma samples and feces samples were composed of methanol-water-36% acetic acid (396.90.1, v/v) and the mobile phase for urine samples was a mixture of methanol-water-36% acetic acid (1089.90.1, v/v). The eluate was monitored by UV absorbance at 260 nm. After a single ig dose of 100 mg x kg(-1) IFM in rats, 5-Fu was mainly distributed in stomach, small intestine, and liver. The concentrations of 5-fluorouracil in other tissues and plasma were low. The excretion of 5-Fu in urine and feces amounted to 0.0065% and 0.063% of the dose, respectively. The method is shown to be accurate and specific, and suitable for preclinical pharmacokinetic studies of IFM.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Urine
/
Pharmacokinetics
/
Prodrugs
/
Tissue Distribution
/
Random Allocation
/
Chemistry
/
Anti-Inflammatory Agents, Non-Steroidal
/
Indomethacin
/
Sensitivity and Specificity
/
Rats, Wistar
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2008
Type:
Article
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