LC-MS/MS determination of budesonide in dog plasma / 药学学报
Acta Pharmaceutica Sinica
; (12): 76-80, 2008.
Article
in Zh
| WPRIM
| ID: wpr-268168
Responsible library:
WPRO
ABSTRACT
A liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method was developed for the determination of budesonide in dog plasma. Budesonide and the internal standard triamcinolone acetonide were separated from plasma by alkalinized liquid-liquid extraction with ethyl acetate. Chromatographic separation was performed on a Capcell Pak C18 MG column with the mobile phase consisted of acetonitrile -5 mmol x L(-1) ammonium acetate (60:40, v/v) at a flow-rate of 0.50 mL x min(-1). A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the negative ion mode. Quantification was performed using multiple reaction monitoring (MRM) of the transitions m/z 489 --> m/z 357 and m/z 493 --> m/z 413 for budesonide and the internal standard, respectively. The linear calibration curves were obtained in the concentration range of 25.0-2000 pg x mL(-1). The lower limit of quantification was 25.0 pg x mL(-1). The intra- and inter-day relative standard deviation over the entire concentration range was less than 15%. The accuracy was in the range of -8.1% to -1.7% in terms of relative error. The method was applied to a pharmacokinetic study of budesonide controlled-release capsules in Beagle dogs. Maximal budesonide plasma level was observed after (3.5 +/- 3.3) h and the Cmax was (786 +/- 498) pg x mL(-1) after a single oral administration of 9 mg budesonide capsules, Cmax was increased to (2142 +/- 1515) pg x mL(-1) after multiple oral administration (9 mg x 5 d) of budesonide capsules. This method was selective and rapid, and the sensitivity was sufficient for the purpose of the pharmacokinetic study of budesonide controlled-release formulation.
Full text:
1
Index:
WPRIM
Main subject:
Blood
/
Pharmacokinetics
/
Chromatography, Liquid
/
Sensitivity and Specificity
/
Area Under Curve
/
Budesonide
/
Spectrometry, Mass, Electrospray Ionization
/
Delayed-Action Preparations
/
Tandem Mass Spectrometry
/
Methods
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2008
Type:
Article