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Isolation and activity of an alpha-amylase inhibitor from white kidney beans / 药学学报
Acta Pharmaceutica Sinica ; (12): 1282-1287, 2007.
Article in English | WPRIM | ID: wpr-268191
ABSTRACT
An alpha-amylase inhibitor (alpha-AI) was isolated from white kidney beans (Phaseolus vulgaris L) by ethanol fractional precipitation, ion exchange chromatography and gel filtration column chromatography. It was a homogeneity glycoprotein demonstrated by SDS-PAGE and gel filtration on CL-6B. The glycoprotein contained 88.2% protein and was rich in aspartic acid, glutamic acid, leucine, threonine and serine. The carbohydrate moiety was consisted of Man, Glc, Gal and Xyl in a mole ratio of 2.42 1.50 1.52 1.00. The glycan and the core protein backbone was connected by O-linkage as determined by beta-elimination reaction. The continuous oral administration of the alpha-AI (150 mg x kg(-1) x d(-1)) for 7 days can lower fasting blood glucose and 300 mg x kg(-1) x d(-1) alpha-AI for 7 days can improve the sugar tolerance on alloxan-dependent diabetic model rats. The result showed the alpha-AI obtained from white kidney beans had good hypoglycemic effect on alloxan induced diabetic rats and may have high potential pharmaceutical value as a regulative digestive-starch degradation in patients suffering from diabetes.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Plants, Medicinal / Plant Proteins, Dietary / Blood / Blood Glucose / Glycoproteins / Chemistry / Rats, Sprague-Dawley / Phaseolus / Plant Lectins Limits: Animals Language: English Journal: Acta Pharmaceutica Sinica Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Plants, Medicinal / Plant Proteins, Dietary / Blood / Blood Glucose / Glycoproteins / Chemistry / Rats, Sprague-Dawley / Phaseolus / Plant Lectins Limits: Animals Language: English Journal: Acta Pharmaceutica Sinica Year: 2007 Type: Article