Isolation and activity of an alpha-amylase inhibitor from white kidney beans / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1282-1287, 2007.
Article
in English
| WPRIM
| ID: wpr-268191
ABSTRACT
An alpha-amylase inhibitor (alpha-AI) was isolated from white kidney beans (Phaseolus vulgaris L) by ethanol fractional precipitation, ion exchange chromatography and gel filtration column chromatography. It was a homogeneity glycoprotein demonstrated by SDS-PAGE and gel filtration on CL-6B. The glycoprotein contained 88.2% protein and was rich in aspartic acid, glutamic acid, leucine, threonine and serine. The carbohydrate moiety was consisted of Man, Glc, Gal and Xyl in a mole ratio of 2.42 1.50 1.52 1.00. The glycan and the core protein backbone was connected by O-linkage as determined by beta-elimination reaction. The continuous oral administration of the alpha-AI (150 mg x kg(-1) x d(-1)) for 7 days can lower fasting blood glucose and 300 mg x kg(-1) x d(-1) alpha-AI for 7 days can improve the sugar tolerance on alloxan-dependent diabetic model rats. The result showed the alpha-AI obtained from white kidney beans had good hypoglycemic effect on alloxan induced diabetic rats and may have high potential pharmaceutical value as a regulative digestive-starch degradation in patients suffering from diabetes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
/
Plant Proteins, Dietary
/
Blood
/
Blood Glucose
/
Glycoproteins
/
Chemistry
/
Rats, Sprague-Dawley
/
Phaseolus
/
Plant Lectins
Limits:
Animals
Language:
English
Journal:
Acta Pharmaceutica Sinica
Year:
2007
Type:
Article
Similar
MEDLINE
...
LILACS
LIS