Effect of etoposide on allergic contact dermatitis induced by dinitrofluorobenzene and its action mechanism in mice / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1050-1053, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-268235
ABSTRACT
This study is to observe the inhibition of etoposide on allergic contact dermatitis (ACD) and explore its possible mechanism of action. Dinitrofluorobenzene was used to induce the allergic contact dermatitis in mouse ear. Three groups of animals were orally administrated with different doses of VP-16 (5, 10, and 20 mg x kg(-1)), separately, for six days. The degree of skin inflammatory reaction was observed by optical microscope. Expression of intercellular adhesion molecule (ICAM-1) was detected by immunohistochemical staining. Radioimmunoassay was applied to measure the serum level of tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10). VP-16 significantly decreased inflammatory cell infiltration and the degree of infiltration reaction, and decreased the level of TNF-a in serum and the expression of ICAM-l in skin. VP-16 can significantly inhibit allergic contact dermatitis induced by DNFB. This therapeutic effect of VP-16 on murine ACD may be due to inhibiting expression of some cytokines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Skin
/
Blood
/
Random Allocation
/
Tumor Necrosis Factor-alpha
/
Interleukin-10
/
Dermatitis, Allergic Contact
/
Intercellular Adhesion Molecule-1
/
Dinitrofluorobenzene
/
Etoposide
Limits:
Animals
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2007
Type:
Article
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