Resolvin E1 protects against ox-LDL-induced injury on vascular endothelial cells / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 1039-1043, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-268261
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether Resolvin E1 (RvE1) could protect against ox-LDL-induced injury on human vein vascular endothelial cells and reveal related molecular mechanisms.</p><p><b>METHODS</b>Human vein vascular endothelial cells were randomly assigned to six groups, which were treated with saline, RvE1, wortmanin, ox-LDL, ox-LDL and RvE1, ox-LDL and RvE1 and wortmanin, respectively. After 48 h, survival rates were determined by MTT, apoptosis rate of cells were determined by flow cytometry, TNF-α contents were assayed by ELISA, caspase 3 and 9 activities were measured by microplate reader, and the expression of p-AKT and LOX-1 were determined by Western blot.</p><p><b>RESULT</b>Compared with normal saline group, survival rate was markedly decreased and apoptosis rate, TNF-α content, caspase 3 and 9 activities, and the expression of LOX-1 were significantly increased in ox-LDL group (P < 0.01). Survival rate was significantly increased and apoptosis rate, TNF-α content, caspase 3 and 9 activities, and the expression of LOX-1 were significantly decreased in ox-LDL + RvE1 group compared to ox-LDL group (P < 0.01), these beneficial effects of RvE1 could be blocked by PI3K inhibitor wortmanin (P < 0.05).</p><p><b>CONCLUSION</b>The present data showed that RvE1 could effectively protect against ox-LDL-induced endothelial cell injury, which might be mediated by PI3K-AKT signaling pathway.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Umbilical Veins
/
Signal Transduction
/
Cells, Cultured
/
Eicosapentaenoic Acid
/
Apoptosis
/
Cell Biology
/
Endothelial Cells
/
Lipoproteins, LDL
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2011
Type:
Article
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