Osteogenic potential of rabbit dermal fibroblasts cultured in vitro: a scanning electron microscopic study / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 75-78, 1999.
Article
in English
| WPRIM
| ID: wpr-268461
ABSTRACT
OBJECTIVE:
To investigate further into the osteogenic potential of rabbit dermal fibroblasts with scanning electron microscope.METHODS:
Split-thickness rabbit skin was processed into small chips that were cultured in vitro and then subjected to scanning electron microscopic study.RESULTS:
The fibroblasts swam out from the skin chips and they increased in number rapidly and became confluent. The cells exhibited squamous configuration, possessing arboreal bifurcation and forming multi-layer structure. The fibroblasts then excreted numerous minute granules, heaping up on and around the cells. Thenceforth emerged on the cell surface fine needle-like crystals, that agglomerated with the granules to form nodules. The fibroblasts orientated themselves in a radiating pattern around the large nodules. Neighboring nodules could be linked up into trabecular structures.CONCLUSIONS:
Sequence of events of new bone formation by rabbit dermal fibroblasts cultured in vitro is fully depicted and confirmed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
1999
Type:
Article
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