Expression and cellular localization of interleukin-8 mRNA and protein in the area of xenogenic bone implant / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 3-7, 1999.
Article
in English
| WPRIM
| ID: wpr-268477
ABSTRACT
OBJECTIVE:
To observe the expression and cellular localization of interleukin-8 (IL-8) mRNA and protein in the area of xenogenic bone implant.METHODS:
The bovine cancellous bone granules were implanted into the thigh muscles of mice. The samples were taken 4, 7, 14 and 21 days after implantation. IL-8mRNA and protein in the site of implant were assayed by in situ hybridization and immunocytochemical techniques.RESULTS:
The expression of IL-8mRNA and protein were observed in all specimens 4, 7, 14 and 21 days after implantation. IL-8mRNA was expressed mainly by the neutrophils, monocytes, macrophages and fibroblasts at 7th day post-implantation. Some mesenchymal cells, multinucleated giant cells, vascular endothelial cells and smooth muscle cells also expressed IL-8mRNA in the area of xenogenic bone implant at 14th and 21st days. Immunocytochemical studies demonstrated the same results as that of in situ hybridization.CONCLUSIONS:
Many different kinds of cells express IL-8mRNA and secret IL-8 in the area of xenogenic bone implant, suggesting that IL-8 may play an important role in local immunity of xenogenic bone graft.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
1999
Type:
Article
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