Chromatographic fingerprint of Cnidium monnieri / 药学学报
Acta Pharmaceutica Sinica
;
(12): 877-881, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-268561
ABSTRACT
To establish the method of HPLC-fingerprint analysis for the quality control of Cnidium monnieri L. Cuss., and identify its active constituents by HPLC-MS, 35 batches of samples were analyzed on a Shimadzu C18 column with a gradient of acetonitrile and 0.1% aqueous aceticacid at a flow rate of 1.0 mL x min(-1) and detected at 245 nm and 322 nm. Furthermore, the typical samples were detected by HPLC-DAD-MS under negative ion mode. 35 batches of Cnidium monnieri L. Cuss. samples were classified into four types based on the results of similarity analysis. According to the comparison of the t(R), MS data and UV maximum absorbance (lambda(max)) values with the standards, 8, 7, 4 and 2 coumarins components were identified in four types of Cnidium monnieri L. Cuss. extracts, separately. The method is repeatable and reliable, and it is capable of effectively controlling the quality of Cnidium monnieri L.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plants, Medicinal
/
Quality Control
/
Seeds
/
Spectrophotometry, Ultraviolet
/
Chemistry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Ecosystem
/
Coumarins
Type of study:
Diagnostic study
/
Prognostic study
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2007
Type:
Article
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