Your browser doesn't support javascript.
loading
The entrapped efficiency of BSA liposome / 药学学报
Acta Pharmaceutica Sinica ; (12): 545-549, 2007.
Article in Chinese | WPRIM | ID: wpr-268600
ABSTRACT
BSA liposomes were prepared with approximately 100 nm mean particle size under rather gentle experiment conditions, and two-colorimetric coomassie brilliant blue protein was employed to measure the free drug in the entrapped efficiency (EE%) determination of BSA liposomes. Gel filtration was used to measure the EE%, and several Sephadex gels were examined by the separation of liposomes and free drug. To determine the free drug, three methods were compared on two-colorimetric UV spectrophotography, Bradford and two-colorimetric coomassie brilliant blue, separately. Two-colorimetric coomassie brilliant blue process increased the accuracy and improved the sensitivity of the assay about 20-fold comparing with the Bradford method. Two-colorimetric coomassie brilliant blue assay appeared to be more sensitive and showed broader dynamic range to measure the free BSA in the EE% determination of BSA liposome.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Particle Size / Rosaniline Dyes / Spectrophotometry, Ultraviolet / Serum Albumin, Bovine / Drug Carriers / Electrophoresis, Gel, Two-Dimensional / Colorimetry / Drug Compounding / Liposomes Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2007 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Particle Size / Rosaniline Dyes / Spectrophotometry, Ultraviolet / Serum Albumin, Bovine / Drug Carriers / Electrophoresis, Gel, Two-Dimensional / Colorimetry / Drug Compounding / Liposomes Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2007 Type: Article