The entrapped efficiency of BSA liposome / 药学学报
Acta Pharmaceutica Sinica
;
(12): 545-549, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-268600
ABSTRACT
BSA liposomes were prepared with approximately 100 nm mean particle size under rather gentle experiment conditions, and two-colorimetric coomassie brilliant blue protein was employed to measure the free drug in the entrapped efficiency (EE%) determination of BSA liposomes. Gel filtration was used to measure the EE%, and several Sephadex gels were examined by the separation of liposomes and free drug. To determine the free drug, three methods were compared on two-colorimetric UV spectrophotography, Bradford and two-colorimetric coomassie brilliant blue, separately. Two-colorimetric coomassie brilliant blue process increased the accuracy and improved the sensitivity of the assay about 20-fold comparing with the Bradford method. Two-colorimetric coomassie brilliant blue assay appeared to be more sensitive and showed broader dynamic range to measure the free BSA in the EE% determination of BSA liposome.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Particle Size
/
Rosaniline Dyes
/
Spectrophotometry, Ultraviolet
/
Serum Albumin, Bovine
/
Drug Carriers
/
Electrophoresis, Gel, Two-Dimensional
/
Colorimetry
/
Drug Compounding
/
Liposomes
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2007
Type:
Article
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