Construction of a prokaryotic expression vector for apoptin and preparation of polyclonal antibody of apoptin / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1405-1407, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-268747
ABSTRACT
<p><b>OBJECTIVE</b>To construct a prokaryotic expression vector for apoptin and prepare polyclonal antibody of apoptin.</p><p><b>METHODS</b>Apoptin gene amplified from pGEM-T/Apoptin plasmid by PCR was cloned into pET-28a (+). E.coli BL21 (DE3) was transformed by the recombinant plasmid, and apoptin protein expression induced by IPTG was analyzed by SDS-PAGE. BALB/c mice were immunized with the protein and the titer of the antibody was determined using indirect enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>Apoptin gene was successfully cloned into pET-28a (+), and the expression of a protein with relative molecular mass of about 17 000 was identified by SDA-PAGE. After 5 immunizations of the mice with the protein, the blood antibody titer reached 15x10(5).</p><p><b>CONCLUSION</b>The prokaryotic expression vector for apoptin is successfully constructed and the polyclonal antibody of apoptin is obtained, which allows further functional study of apoptin.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Blood
/
Gene Expression
/
Genome
/
Cloning, Molecular
/
Capsid Proteins
/
Allergy and Immunology
/
Escherichia coli
/
Genetic Vectors
/
Genetics
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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