Construction of delta-pIRES2-EGFP plasmid and its expression in HEK293 cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1351-1353, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-268762
ABSTRACT
<p><b>OBJECTIVE</b>To construct the delta-pIRES2-EGFP plasmid and investigate its expression in HEK293 cells.</p><p><b>METHODS</b>Full length cDNA of rat delta opioid receptor gene amplified from rat brain tissues using reverse transcription and nested PCR was cloned into pMD20 T vector. The delta cDNA was inserted into pIRES2-EGFP plasmid to construct the recombinant eukaryotic plasmid delta-pIRES2-EGFP, which was transfected into HEK293 cells via Lipofectamine2000. The expression of delta was examined under fluorescence microscope.</p><p><b>RESULTS</b>The recombinant delta-pIRES2-EGFP plasmid was successfully constructed, and high expression of delta was detected in HEK293 cells transfected by the plasmid.</p><p><b>CONCLUSION</b>delta-pIRES2-EGFP has been successfully cloned, which shows high expression of delta in HEK293 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Recombinant Fusion Proteins
/
Transfection
/
Gene Expression
/
Polymerase Chain Reaction
/
Rats, Sprague-Dawley
/
DNA, Complementary
/
Receptors, Opioid, delta
/
Green Fluorescent Proteins
/
HEK293 Cells
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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