Effect of small interfering RNA targeting CXCR4 on breast cancer angiogenesis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 954-958, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-268800
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of a small interfering RNA targeting CXCR-4 (shRNA-CXCR4) on angiogenesis of human breast cancer cells.</p><p><b>METHODS</b>The expression of CXCR4 mRNA and protein in 3 breast cancer cell lines with CXCR-4 silencing mediated by shRNA-CXCR4 was detected by RT-PCR and Western blotting, respectively. The morphological changes of human umbilical vein endothelial cells (HUVECs) were observed in co-culture with human breast cancer cells after CXCR4 gene silencing.</p><p><b>RESULTS</b>CXCR4 mRNA and protein expressions decreased significantly in MCF-7, MDA-MB-231 and MDA-MB-435s breast cancer cells after the gene silencing (P<0.05). Gene silencing with shRNA-CXCR4 in human breast cancer cells significantly inhibited the ability of HUVECs to form tubular structures in the co-culture (P<0.05).</p><p><b>CONCLUSION</b>Gene silencing by shRNA-CXCR4 can obviously lower the angiogenesis-inducing ability of human breast cancer cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Umbilical Veins
/
Breast Neoplasms
/
RNA, Messenger
/
Coculture Techniques
/
Receptors, CXCR4
/
Cell Biology
/
RNA, Small Interfering
/
RNA Interference
/
Cell Line, Tumor
/
Endothelial Cells
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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