Effect of sequential intratumoral injection of xenogeneic antigens for immunotherapy in immunized mice bearing S180 tumor / 南方医科大学学报
Journal of Southern Medical University
;
(12): 856-863, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-268827
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the therapeutic effect of sequential intratumoral injection of xenogeneic antigens in immunized tumor-bearing mice.</p><p><b>METHODS</b>Sequential intratumoral injection of the xenoantigens was performed in immunized mice bearing S180 tumor. The tumor size changes were observed, and the tumor-infiltrating lymphocytes (TIL) including CD3+CD4+T, CD3+CD8+T, and CD3+CD4+CD25+T lymphocytes were counted with flow cytometry. The concentrations of IL-2 and TNF-alpha in the tumor was measured using ELISA.</p><p><b>RESULTS</b>No significant difference was found in the number of CD3+T lymphocytes in the TILs between different groups. After the immunotherapy, the percentages of CD3+CD4+T, CD3+CD8+T and CD3+CD4+CD25+T lymphocytes were 54%, 22% and 2.91%, respectively, with the CD4+/CD8+ ratio of 2.49, significantly different from that in the control group (P<0.05). The concentrations of IL-2 and TNF-alpha were 100.61 pg/ml and 54.114 pg/ml, respectively, significantly different from those in the control group (P<0.05).</p><p><b>CONCLUSION</b>Sequential intratumoral injection of heteragenetic antigena can significantly increase the amount of effector cells and cytokines in the micro-environment of the tumor, and decrease the expression of T regulatory.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sarcoma 180
/
Streptococcus
/
Therapeutics
/
Random Allocation
/
Antigens, Heterophile
/
Lymphocytes, Tumor-Infiltrating
/
CD4-CD8 Ratio
/
Cell Biology
/
Allergy and Immunology
/
Immunotherapy
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2009
Type:
Article
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