Etiologic investigation of chronic granulomatous inflammation of hand by polymerase chain reaction and DNA sequencing / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 669-671, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-268870
ABSTRACT
<p><b>OBJECTIVE</b>To explore the causative pathogens in littoral hand infections which exhibited chronic granulomatous inflammation, the relationship between chronic granulomatous inflammation and mycobacteria and to discuss the prospects of PCR in clinical application for diagnosis of granulomatous inflammation.</p><p><b>METHOD</b>With 16S-rDNA as the target sequence, Nest-PCR was used to detect mycobacteria directly from 37 cases of chronic granulomatous inflammations, and identified them by gene sequencing.</p><p><b>RESULTS</b>Twenty-four of 37 cases were positive for mycobacteria by Nest-PCR, in which 17 were M.marinum, 1 M.chelonae, 2 M.avium, 2 M.kansasii, and 2 M.tubercular through gene sequencing.</p><p><b>CONCLUSIONS</b>Nest-PCR combining gene sequencing proved to be a liable and sensitive method to detect Non-tubercular mycobacteria (NTM) in fresh tissue. NTM is the major factor of hand specific chronic infections other than tubercular. Pathological changes are difficult to differentiate TB from NTM and bacterial evidence was necessary.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Bacterial
/
RNA, Ribosomal, 16S
/
Chemistry
/
Polymerase Chain Reaction
/
Chronic Disease
/
Sequence Analysis, DNA
/
Mycobacterium marinum
/
Molecular Diagnostic Techniques
/
Diagnosis
/
Genetics
Type of study:
Diagnostic study
/
Etiology study
/
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2006
Type:
Article
Similar
MEDLINE
...
LILACS
LIS