Study of mRNA expression of vascular endothelial growth factor-(A, C, D) genes and its effect on prognosis of breast cancer / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 473-477, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-268930
ABSTRACT
<p><b>OBJECTIVE</b>To study mRNA expression of VEGF-(A, C, D) genes in breast carcinoma and its effect on prognosis.</p><p><b>METHODS</b>The mRNA expression of VEGF-(A, C, D) genes in 61 cases of primary breast carcinoma and 29 cases of benign mammary lesions was detected by TaqMan real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) technology. The correlation between mRNA expression and various clinicopathologic parameters and survival data was analyzed.</p><p><b>RESULTS</b>The level of mRNA expression of VEGF-(A, C) genes was significantly higher in primary breast carcinomas (2.79 +/- 1.31 and 3.33 +/- 0.88 respectively) than in benign mammary lesions (1.59 +/- 1.35 and 2.76 +/- 0.55 respectively, P = 0.000 and 0.002 respectively). The percentage of VEGF-D mRNA expression was 73.77% in breast carcinomas, as compared to 51.72% in benign mammary lesions (P = 0.038). However, there was no statistically significant difference in the mRNA expression levels of VEGF-D gene between primary breast carcinomas and benign mammary lesions (P = 0.683). On the other hand, the ratio of VEGF-D mRNA expression to VEGF-C mRNA expression was lower in lymph node-positive than in lymph node-negative cases. This also correlated with the occurrence of nodal metastasis by uni- and multivariate analysis (P(uni) = 0.046 and P(mult) = 0.062). High mRNA expression of VEGF-(A, C) genes was associated with poor disease-free survival (P = 0.030 and 0.044 respectively).</p><p><b>CONCLUSIONS</b>The expression of VEGF-(A, C, D) genes may play a role in the disease progression of breast carcinoma. The ratio of VEGF-D mRNA expression to VEGF-C mRNA expression correlates with the occurrence of lymph node metastasis in breast carcinoma. The mRNA expression of VEGF-(A, C) genes may serve as a useful prognostic indicator in breast cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Prognosis
/
Breast Neoplasms
/
RNA, Messenger
/
Gene Expression Regulation, Neoplastic
/
Follow-Up Studies
/
Reverse Transcriptase Polymerase Chain Reaction
/
Vascular Endothelial Growth Factor A
/
Vascular Endothelial Growth Factor C
/
Vascular Endothelial Growth Factor D
Type of study:
Observational study
/
Prognostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2006
Type:
Article
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